General Cell Lines and Cell Cultures
Lonza Walkersville Primary Cell Optimization 96-well Nucleofector Kit
The Primary Cell Optimization Nucleofector Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector 96-well Unit.Applications:Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector 96-well UnitContent:0.675 mL Nucleofector Solution P10.675 mL Nucleofector Solution P20.675 mL Nucleofector Solution P30.675 mL Nucleofector Solution P40.675 mL Nucleofector Solution P54 x 0.15 mL Supplement 11 x 0.15 mL Supplement 32 x 50 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0) 2 x 96-well Nucleocuvette Plates
Genscript Corporation CHO-K1/cyno CTLA4 Stable Cell
CTLA4 or CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) also known as CD152 (cluster of differentiation 152) is a protein receptor that functioning as an immune checkpoint downregulates immune responses CTLA4 is constitutively expressed in Tregs but only upregulated in conventional T cells after activation It acts as an
Genscript Corporation CHO-K1/CD64 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress high affinity human Fc fragment of IgG receptor Ia (Fc RI CD64) on the cell surface containing one alpha chain and two gamma chains The surface expression of CD64 is validated by FACS analysis This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies Fc fusion proteins or antibody based biologics that bind with CD64 during biologics research and development
Genscript Corporation CHO-K1/CMKLR1/G15 Stable Cell
The orphan receptor ChemR23 (also referred to as CMKLR1 or DEZ) is a G-protein coupled receptor (GPCR) with homology to neuropeptide and chemoattractant receptors ChemR23 is also a G i-linked receptor expressed primarily by monocytes M s and plasmacytoid dendritic cells CMKLR1 has been shown to be expressed by DC generated in vitro from monocytes to mediate their migration to the proteolytically regulated chemoattractant chemerin and CMKLR1 may be a key mediator of pDC recruitment from the blood into tissue sites enriched in active chemerin
Genscript Corporation CHO-K1/D2s/G15 Stable Cell Li
The short form of D2 (D2S) and the long form (D2L) are two isoforms that contribute differentially to dopamine signaling in both prefrontal cortex and striatum The D2 dopamine receptor short form (D2s) has been shown to stimulate phospholipase D (PLD) activity independent of the activation of phospholipase C (PLC) activity in GH4 derived cells stably transfected with the D2s receptor Agonist activation of D2s has been shown to mediate the inhibition of growth in the same cell line
Genscript Corporation CHO-K1/D5/G15 Stable Cell Lin
Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain where it controls a variety of functions including locomotor activity cognition emotion positive reinforcement food intake and endocrine regulation It also plays multiple roles in the periphery as a modulator of cardiovascular function catecholamine release hormone secretion vascular tone renal function and gastrointestinal motility The dopamine receptor family consists of five members which are classified into two groups D1-like (D1 and D5) and D2-like (D2 D3 and D4) D5 receptor has a 10-fold higher affinity for dopamine than the D1 receptor
Genscript Corporation CHO-K1/CD38 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human CD38 on the surface The surface expression of CD38 is validated by FACS analysis
Genscript Corporation CHO-K1/MTLR/G15 Stable Cell L
The motilin receptor (MTLR) represents a clinically useful pharmacological target as agonists binding to the MTLR have gastroprokinetic properties Motilin is a 22 amino acid peptide that potently stimulates gastrointestinal contractility The biological effects of motilin are mediated by a G sub q /sub -coupled seven transmembrane protein currently termed motilin receptor (MR) that shares significant sequence similarity with the ghrelin receptor The motilin receptor is also activated by the antibiotic erythromycin this interaction appears to mediate some of the gastrointestinal side effects of erythromycin Although motilides (non-antibiotic derivatives of erythromycin) such as ABT-229 have been investigated for treatment of diabetic gastroporesis the effectiveness has been limited by tachyphylaxis (decreased response to ligand) resulting from receptor downregulation Agonists of the motilin receptor with reduced densensitization activity remain a potential treatment for dis
Genscript Corporation CHO-K1/OX40 Stable Cell Line
OX40 (CD134) is a member of the TNFR/TNF superfamily Costimulatory signals from OX40 to a conventional T cell promotes division and survival augmenting the clonal expansion of effector and memory populations as they are being generated to antigens OX40 additionally suppresses the differentiation and activity of Treg further amplifying this process
Genscript Corporation HEK293/EP1 Stable Cell Line
Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein G q to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/ADRB1/G15 Stable Cell
The -adrenergic receptors are linked to G proteins The -receptor has three known subtypes Beta-1 receptors primarily regulate myocardial tissue and affect the rate of contraction via impulse conduction Beta-2 receptors regulate smooth muscle tone and influence vascular and bronchiolar relaxation Beta-3 receptors are less well studied but are thought to primarily affect lypolysis and may have effects on cardiac inotropy (Greene Shepherd 2006) In the human heart beta (1)- and beta (2)AR are the most powerful physiologic mechanism to acutely increase cardiac performance Changes in betaAR play an important role in chronic heart failure (CHF) Thus due to increased sympathetic activity in CHF betaAR are chronically (over) stimulated and that results in beta (1) AR desensitization and alterations of down-stream mechanisms (Brodde OE 2006)
Genscript Corporation CHO-K1/CRF2/G15 Stable Cell L
Recombinant CHO-K1 cells stably overexpress human corticotropin releasing hormone receptor 2 (CRF2) on the surface and contain high levels of G protein G s to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/cyno PD-L2 Stable Cell
Programmed cell death 1 ligand 2 (PD-L2) is a protein that is encoded by the PDCD1LG2 gene in humans PDCD1LG2 has also been designated as CD273 (cluster of differentiation 273) Inhibitory molecules of the B7/CD28 family play a key role in the induction of immune tolerance in the tumor microenvironment The programmed death-1 receptor (PD-1) with its ligands PD-L1 and PD-L2 constitutes an important member of these inhibitory pathways PD-L2 expression was initially thought to be restricted to antigen-presenting cells such as macrophages and dendritic cells (DCs) However PD-L2 expression can be induced on a wide variety of other immune cells and nonimmune cells depending on micro environmental stimuli
Genscript Corporation CHO-K1/5-HT1A/G15 Stable Cell
The 5-Hydroxytryptamine receptor 1A (5-HT1A) is G sub i /sub -coupled GPCRs expressed in the cerebral cortex hippocampus septum amygdala and raphe nucelus with lesser amounts in the basal ganglia and thalamus Many functions of the central nervous system are influenced by 5-HT including sleep motor activity sensory perception arousal and appetite 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression anxiety and schizophrenia
Genscript Corporation CHO-K1/VPAC2/G15 Stable Cell
Recombinant CHO-K1 cells stably overexpress human vasoactive intestinal peptide receptor 2 (VPAC2) on the surface and contain high levels of G protein G s to couple with the receptor in downstream signaling pathways